In vivo evaluation of corneal wound healing in superficial keratectomy with the tandem scanning confocal microscope

Authors

  • Sek-jin Chew LSU Eye Center, New Orleans, LA, USA
  • Dennis SC Lam Chinese University of Hong Kong
  • Roger W Beuerman Chinese University of Hong Kong
  • Marguerite B McDonald Chinese University of Hong Kong
  • Herbert E Kaufman Chinese University of Hong Kong

Abstract

Aim: An in vivo study with a tandem scanning confocal microscope (TSM) on the process of keratocyte activation, proliferation, and stromalfibrosis after superficial keratectomy, and nylon suture penetration of rabbit cornea.

Materials and methods: A TSM which would provide improved resolution, magnifiction and contrast, as well as serial optical sectioning ability, was used for real-time examination of the rabbit cornea following superficial keratectomy. Additionally, the effects of nylon suture penetration and stromal revascularization were studied.

Results: Despite an apparently stable slit lamp appearance, with minimal subeoithelial fibrosis, the TSM revealed an actively evolving process of collagen remodelling in the anterior stroma. A suture placed in the stroma was also observed to produce significant tractional forces and tissue displacement. Subsequent stromal fibroblast activation and fibrosis around the nylonwas also observed.

Conclusion : Our results suggest that TSM is a useful and effective instrument in evaluating corneal stromal wound healing at the cellular level.

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Published

1997-03-01

How to Cite

1.
Chew S- jin, Lam DS, Beuerman RW, McDonald MB, Kaufman HE. In vivo evaluation of corneal wound healing in superficial keratectomy with the tandem scanning confocal microscope. Hong Kong J Ophthalmol [Internet]. 1997Mar.1 [cited 2021Feb.25];1(1):34-40. Available from: https://hkjo.hk/index.php/hkjo/article/view/169

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Section

Original Articles